Updated: May 5, 2015

AxioPlan Fluorescence Microscope

epif

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INSTRUCTIONS

Epifluorescence microscope is usally a compound microscope equipped with epifluorescent light path system, such as the Zeiss Axioplan  in the following illustrations.  The computer-assisted digital imaging operations greatly facilitate the users for acquiring images both in transmitted or fluorescent light condition.


1. Transmitted light (bright field, BF) microscopy

2. Differential interference contrast (DIC) microscopy

DIC microscopy uses polarized transmitted light to increase the contrast of images of unstained cells. This was developed by Nomarski, and is also called Nomarski contrast. Briefly, the specimen is illuminated with plane polarized light, and after passing through the specimen and the objective, the light passes through a second polarizing filter that is at an angle to the first. The polarity of the light is changed as it passes through the specimen, leading to increased contrast and the appearance of a three-dimensional image. There are four parts to the optical system:dic

 

3. Epifluorescence microscopy

* Taking pictures with the digital camera-- for detailed information, please see ZEN lite guide.



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Changing mercury bulb