Glass Knife Maker
- Choice of 25 mm and 38 mm glass strips.
- Felxible scoring selection.
- Preparing glass knives for trimming or cutting thin/ultrathin sections of embedded TEM specimens.
strip of glass with soapy water and/or alcohol to remove grease and
dirt and dry on clean lint-free paper. For subsequent handling, hold
the strip with a Kimwipe.
- If the glass strip is new (full
sized), break it in half first. To cut strip exactly in half, remove
fork and place strip over two pegs so that right end is at dot. The
rippled (serrated) edge of the strip should be placed face down.
To make glass square:
the glass bar so that the end is resting against the arresting stud and
the side is against the angle setting plate. The angle setting plate
should be set at 80° or 90°.
- Hold and lower clamping head
onto strip with locking lever and tighten slightly to secure glass in a
level position. Do not bounce the scorer on the glass.
- Select scoring position to parallel (marked with lines).
glass by pulling scoring wheel across glass from back to front with
continuous smooth stroke. Score should be distinct but not heavy enough
to produce splinters of glass.
- Break glass by turning breaking knob clockwise slowly until glass begins to break.
- After glass breaks, turn breaking knob fully counter-clockwise to initial position.
- Lift clamping head, assisting with your hand, push scoring shaft back in, and remove the glass square with fork.
To make glass knives:
the glass squire ¼ turn counterclockwise (see figure on the machine),
put on top of breaking pins by inserting lower left edge between white
guiding rings of front glass holder. Lower rear glass holder onto upper
left edge of square by pushing in and turning counter-clockwise to
disengage the knob.
- Select scoring position marked with a diamond and 25 for 25mm glass.
- Lower the head, score, break and remove the glass as before.
marks should be no more than 0.2 mm away from the upper left and lower
right edges of center line. The upper edge of the left triangle and the
lower edge of the right triangle are the knives. These edges should not
To check knives:
the glass knife with a dissecting scope. Good knife should have a
narrow shoulder (<0.2mm), and as faint a pressure curve as possible.
For either thick or thin sectioning, examine the knife edge directly to
locate any edge imperfections, whiskers, contamination or fingerprints
on the back of the knife. For thin sectioning, bounce light off the
front edge of the knife. Any lines running parallel to the stress
mark which sweeps from the left may result in knife marks. The
best knives do not show this artifact. The sharpest edge is to the left
generally, and the most durable surface is to the right (but left of
To put boats (troughs) on knives:
a commercial plastic boat, or use aluminum tape or electrical tape to
make a boat. Seal with nail polish. Stick on double-sized tape and
stored in a dust-free box. The knives are recommended to be used within
a few days.