A SHORT CURRICULUM VITAE for GORDON, John Robert (PhD)
|
REFEREED PUBLICATIONS (last 5 years)
- F. Shahriar, M. Ngeleka, J.R. Gordon , and E. Simko. Identification by mass spectroscopy of F4-fimbrial-binding proteins in porcine milk and characterization of lactadherin as an inhibitor of F4-positive Escherichia coli attachment to intestinal villi in vitro. Developmental and Comparative Immunology, in press.
- J.R. Gordon , F. Li, X. Zhang, W. Wang, X. Zhao, and A. Nayyar. 2005. The combined CXCR1/CXCR2 antagonist CXCL8 (3-73) K11R/G31P blocks neutrophil infiltration, pyrexia and pulmonary vascular pathology in endotoxemic animals . J Leukocyte Biology, in press . NSERC-funded
- X. Zhang, H. Huang, J. Yuan, D. Sun, W-S. Hou, J.R. Gordon , and Jim Xiang. 2005. CD4 - 8 - dendritic cells prime CD4 + T regulatory 1 cells to suppress antitumor immunity. Jounal of Immunology 175: 2931-2937.
- J.R. Gordon , F Li, J. Xiang, and X. Zhang. 2005. Allergen-presenting splenic CD8-?lpha + dendritic cells tolerize allergic lung disease via an IL-10-dependent, contact-independent, mechanism. Journal of Immunology 175: 1516-1522.
- C. Ebeling, P. Forsyth, P. Ng, J.R. Gordon , N. Hollenberg, and H. Vliagoftis. 2005. Proteinase-activated receptor-2 activation in the airways enhances antigen-mediated airway inflammation and airway hyperresponsiveness through different pathways. J Allergy and Clinical Immunology 115: 623-30.- P. Forsythe, C. Ebeling, JR. Gordon, AD Befus and H Vliagoftis. 2004. Opposing effects of short and long term stress on airway inflammation. American Journal of Respiratory and Critical Care Medicine 169:220-6
- J.R. Gordon, V.A. Swystun, F. Li, X. Zhang, BE Davis, P Hull, and DWC Cockcroft. 2003. Regular salbutamol use increases CXCL8 responses in asthma. Relationship to the eosinophil response. European Respiratory Journal 22:1-9
- F. Li, X. Zhang, C. Mizzi, and J.R. Gordon. 2002. CXCL8(3-73)K11R/G31P antagonizes the neutrophil chemoattractants present in pasteurellosis and mastitis lesions and abrogates neutrophil influx into intradermal endotoxin challenge sites in vivo. Vet Immunol Immunopathol 90:65-77.
- X. Zhang, Z. Chen, H. Huang, J.R. Gordon, and J. Xiang. Analysis of the gene expression profiles of naive and activated tumor-specific T cells. Life Sci 71: 3005-17
- W. Zhang, Z. Chen, F. Li, H. Kamencic, B. Juurlink, J.R. Gordon, and J. Xiang. TNF transgene-expressing dendritic cells undergo augmented cellular maturation, and induce more robust T cell activation and antitumor immunity than dendritic cells generated in recombinant TNF. Immunol, in press.
- Li, F., X. Zhang, and J.R. Gordon. 2002. CXCL8(3-73)K11R/G31P antagonizes ligand binding to the CXCR1 and CXCR2, and neutrophil responses to CXCL8/IL-8. Biochem Biophys Res Comm 293:939-44.
- H Huang, F Li, JR Gordon, and J Xiang. 2002. Synergistic enhancement of anti-tumor immunity with adoptively transferred tumor-specific CD4+ and CD8+ T cells and intratumoral lymphtactin transgene expression. Cancer Res 62:2043-51
- Z. Chen, S. Dehm, K. Bonham, H. Kamencic, B. Juurlink, X.Zhang, J.R. Gordon, and J. Xiang. 2002. DNA array and biological characterization of the impact of the maturation status of mouse dendritic cells on their phenotype and antitumor vaccination efficacy. Cell Immunol 214:60-71.
- Z. Chen, J.R. Gordon, X. Zhang, and J. Xiang. 2002. Analysis of the gene expression profiles of immature and mature bone marrow-derived dendritic cells using DNA arrays. Biochem Biophys Res Comm 290:66-72.
- A.M. Schneider, X. Zhang, F. Li, and J.R. Gordon. 2001. Differential induction of allergen-specific IgA, airway hyperresponsiveness, or allergic airway disease following sensitization with limiting doses of ovalbumin-alum. Cell Imm 212:101.
- F. Li and J.R. Gordon. 2001. IL-8(3-73)K11R is a high affinity agonist of the neutrophil CXCR1 and CXCR2. Biochem Biophys Res Comm 286:595-600.
- JL Caswell, DM Middleton, and JR Gordon. 2001. The importance of interleukin-8 as a neutrophil chemoattractant in the lungs of cattle with pneumonic pasteurellosis. Canad J Vet Res 65: 229-32.
- C.M.Cairns, J.R.Gordon, F.Li, & J. Xiang. 2001. Lymphotactin expression by engineered myeloma tumor cells drives tumor regression: Mediation by CD4+ and CD8+ T cells and neutrophils expressing the XCR1 receptor. J Immunol 167: 57-65.
- H Huang, F Li, CM. Cairns, JR Gordon, and J Xiang. 2001. Neutrophils and B lymphocytes express the XCR1 receptor and chemotactically respond to lymphotactin. Biochem Biophys Res Comm 281:378-82.
- C.L. Theoret, S.M. Barber, & J.R. Gordon. 2001. Temporal localization of immunoreactive transforming growth factor-beta1 in normal equine skin and in full-thickness dermal wounds. Vet Surgery 31:266-73.
- C.L. Theoret, S.M. Barber, T. Moyana & J.R. Gordon. 2001. Preliminary observations on expression of transforming growth factors-beta-1 and -3 in equine full-thickness skin wounds healing normally or with exuberant granulation tissue. Vet Surgery 31:274-80.
- C.L. Theoret, S.M. Barber, T. Moyana and J.R. Gordon. (2001). Expression of transforming growth factor beta-1, beta-3, and basic fibroblast growth factor in full-thickness skin wounds of equine limbs and thorax. Vet Surgery 30:269-77.
- J.R. Gordon, X. Zhang, K. Stevenson and K. Cosford. (2000). Thrombin induces IL-6 but not TNF-alpha expression by mouse mast cells. Threshold-level thrombin receptor and very low level Fc-epsilonRI signaling synergistically enhance IL-6 secretion. Cell Immunol 205: 128 - 35.
- KJ Gaspar, KJ Racette, JR Gordon, ME Loewen and GW Forsyth. (2000). Cloning a chloride conductance mediator from the apical membrane of porcine ileal enterocytes. Physiological Genomics 3: 101 - 11.
- R.S. Kaushik, J.E. Uzonna, D. Radzioch, J.R. Gordon, and H. Tabel. (2000) Differences in cytokine (TNF-alpha, IL-6, IL-10 and IL-12) production by bone marrow-derived macrophages from resistant and susceptible mice. Cytokine 12: 1024 - 34.
- J.R. Gordon. (2000). Monocyte chemoattractant protein-1 (MCP-1) expression cutaneous allergic responses in mice is mast cell-dependent and largely mediates monocyte recruitment. J Allergy Clin Immunol 106: 110 - 116.
- V.A. Swystun, J.R. Gordon, E.E. Davis, X. Zhang, D.W. Cockcroft. (2000). Mast cell tryptase release and asthmatic responses to allergen increase with regular use of salbutamol. J Allergy Clin Immunol 106: 57 - 64.
- J.R. Gordon. (2000) TGF-beta1 and TNF-alpha secreted from mast cells stimulated via the Fc-epsilonRI activate fibroblasts for high level production of monocyte chemoattractant protein-1. Cell Immunol 201: 42 - 49.
- Z. Papp, D.M. Middleton, C.M. Rontved, M. Foldvari, J.R. Gordon, and M.E. Baca-Estrada. (2000) Transtracheal administration of interleukin-12 induces intense neutrophil responses in the murine lung. J Interferon Cytokine Res 20: 191 - 95.
- R.S. Kaushik, J.E. Uzonna, J.R. Gordon, and H. Tabel (I999) Innate resistance
to Trypanosoma congolense infections: Differential production of nitric oxide
by macrophages from susceptible BALB/c and resistant C57B1/6 mice. Exp Parasitol
92: 131-143.
Books, chapters & reviews (last 5 years)
- J. Xiang and J.R. Gordon, 2003. Genetic engineering of a recombinant fusion protein possessing an anti-tumor antibody fragment and a TNF-a moiety. In Methods in Molecular Biology 215: 201-12.
- X. Zhang, J.R. Gordon, J. Xiang. 2003. Advances in dendritic cell-based vaccines
for cancers. Cancer Biotherapy and Radiopharmaceuticals, in press
J.R. Gordon, 2002. A Practical Guide to Cellular and Molecular Research Methods
in Immunology. Fourth edition. 140 pages. University of Saskatchewan
Particularly significant prior papers/reviews/chapters
- X. Zhang, J.R. Gordon, J. Xiang, 2002. Advances in Dendritic Cell-Based Vaccines
for Cancer. Cancer Biother Radiopharmacol 17:601-619.
- J. Xiang and J.R. Gordon, 2002. Genetic Engineering of a Recombinant Fusion
Protein Possessing an Anti-Tumor Antibody Fragment and a TNF Moiety. In Methods
in Molecular Medicine. W. Kiess, D. Korhoz and A. Kosser (eds). Humana Press
Inc, Totowa, NJ. pp 201-12.-J.R. Gordon, 1997. FceRI-induced cytokine production
and gene expression. In: IgE receptor (FceRI) function in mast cells and basophils.
M.M. Hamaway (ed.), R.G. Landes, Austin, Texas. pp 209-242.
- S.J. Galli, J.R. Gordon, B.K. Wershil, A. Elovic, D.T.W. Wong, P.F. Weller,
1994. Mast cell and eosinophil cytokines in allergy and inflammation. In:
Gleich, G.J., Kay A.B., eds. Eosinophils: Immunological and Clinical Aspects,
pp. 255-280. New York: Marcel Decker.
- J.R. Gordon, and S.J. Galli, 1992. Mast cells as a source of multifunctional
cytokines. In: Dammacco, F.(ed.), Advances in Tumor Immunology and Allergic
Disorders, pp. 165-186. London: Springer International.
- S.J. Galli, J.R. Gordon, and B.K. Wershil, 1991. Cytokine production by mast
cells and basophils. Curr Opin Immunol 3: 865-873.
- J.R. Gordon, P.R. Burd, S.J. Galli, 1990. Mast cells as a source of multifunctional
cytokines. Immunol Today 11:458-464.
______________________________________________________________________________________________________________________
- J.R. Gordon, V.A. Swystun, F. Li, X. Zhang, BE Davis, P Hull, and DWC Cockcroft.
2003. Regular salbutamol use increases CXCL8 responses in asthma. Relationship
to the eosinophil response. Eur Resp J 22:1-9.
- F. Li, X. Zhang, C. Mizzi, and J.R. Gordon. 2002. CXCL8(3-73)K11R/G31P antagonizes
the neutrophil chemoattractants present in pasteurellosis and mastitis lesions
and abrogates neutrophil influx into intradermal endotoxin challenge sites
in vivo. Vet Immunol Immunopathol 90:65-77.
- W. Zhang, Z. Chen, F. Li, H. Kamencic, B. Juurlink, J.R. Gordon, and J.
Xiang. 2003. TNF transgene-expressing dendritic cells undergo augmented cellular
maturation, and induce more robust T cell activation and antitumor immunity
than dendritic cells generated in recombinant TNF. Immunol 108:177-88.
- Li, F., X. Zhang, and J.R. Gordon. 2002. CXCL8(3-73)K11R/G31P antagonizes
ligand binding to the CXCR1 and CXCR2, and neutrophil responses to CXCL8/IL-8.
Biochem Biophys Res Comm 293:939-44
- Z. Chen, S. Dehm, K. Bonham, H. Kamencic, B. Juurlink, X.Zhang, J.R. Gordon,
and J. Xiang. 2002. DNA array and biological characterization of the impact
of the maturation status of mouse dendritic cells on their phenotype and antitumor
vaccination efficacy. Cell Immunol 214:60-71
- Z. Chen, J.R. Gordon, X. Zhang, and J. Xiang. 2002. Analysis of the gene
expression profiles of immature and mature bone marrow-derived dendritic cells
using DNA arrays. Biochem Biophys Res Comm 290:66-72
- A.M. Schneider, X. Zhang, F. Li, and J.R. Gordon. 2001. Differential induction
of allergen-specific IgA, airway hyperresponsiveness, or allergic airway disease
following sensitization with limiting doses of ovalbumin-alum. Cell Immunol
212:101-109
- F. Li and J.R. Gordon. 2001. IL-8(3-73)K11R is a high affinity agonist of
the neutrophil CXCR1 and CXCR2. Biochemical & Biophysical Research Communications
286:595-600
- JL Caswell, DM Middleton, and JR Gordon. 2001. The importance of interleukin-8
as a neutrophil chemoattractant in the lungs of cattle with pneumonic pasteurellosis.
Can J Vet Res 65: 229-32
- C.M.Cairns, J.R.Gordon, F.Li, & J. Xiang. 2001. Lymphotactin expression
by engineered myeloma tumor cells drives tumor regression: Mediation by CD4+
and CD8+ T cells and neutrophils expressing the XCR1 receptor. J Immunol 167:
57-65
- H Huang, F Li, CM. Cairns, JR Gordon, and J Xiang. 2001. Neutrophils and
B lymphocytes express the XCR1 receptor and chemotactically respond to lymphotactin.
Biochem Biophys Res Comm 281:378-82.
- J.R. Gordon, X. Zhang, K. Stevenson and K. Cosford. 2000. Thrombin induces
IL-6 but not TNF-alpha expression by mouse mast cells. Threshold-level thrombin
receptor and very low level Fc-epsilonRI signaling synergistically enhance
IL-6 secretion. Cell Immunol 205: 128 - 35
- J.R. Gordon. 2000. Monocyte chemoattractant protein-1 (MCP-1) expression
cutaneous allergic responses in mice is mast cell-dependent and largely mediates
monocyte recruitment. J All Clin Immunol 106: 110 - 116
- V.A. Swystun, J.R. Gordon, E.E. Davis, X. Zhang, D.W. Cockcroft. 2000. Mast
cell tryptase release and asthmatic responses to allergen increase with regular
use of salbutamol. J All Clin Immunol 106: 57 - 64.
- J.R. Gordon. 2000. TGF-beta1 and TNF-alpha secreted from mast cells stimulated
via the Fc-epsilonRI activate fibroblasts for high level production of monocyte
chemoattractant protein-1. Cell Immunol 201: 42 - 49
- Z. Papp, D.M. Middleton, C.M. Rontved, M. Foldvari, J.R. Gordon, and M.E.
Baca-Estrada. 2000. Transtracheal administration of interleukin-12 induces
intense neutrophil responses in the murine lung. J Interfer Cyto Res 20: 191
- 95
- J.L. Caswell, D.M. Middleton, S.D. Sorden, and J.R. Gordon, 1997. Expression
of the neutrophil chemoattractant IL-8 in the lesions of bovine pneumonic
pasteurellosis. Vet Pathol 35: 124-131.
- J.C. Kendall, X.-H. Li, S.J. Galli, and J.R. Gordon, 1997. Promotion of mouse
fibroblast proliferation by IgE-dependent activation of mouse mast cells:
role for TNF and TGF-b. J Allergy Clin Immunol 99:113-123.
- J.R. Gordon, and S.J. Galli, 1994. Promotion of mouse fibroblast collagen
gene expression by mast cells stimulated via the FceRI. Role for mast cell-derived
TGFbeta and TNF. J Exp Med 180: 2027-2037.
- S. Galli, J.R. Gordon, and B.K. Wershil, 1993. Mast cell cytokines in allergy
and inflammation. Agents Actions 43: 209-219.
- J.J. Costa, K. Matossian, W.J. Beil, D.T.W. Wong, J.R. Gordon, A.M. Dvorak,
P.F. Weller, and S.J. Galli, 1993. Human eosinophils can express the cytokines
TNF-a and MIP1a. J Clin Invest 91: 2673-84.
- J.R. Gordon, and S.J. Galli, 1991. Release of both preformed and newly synthesized
TNF by mouse mast cells stimulated via the FceRI. A mechanism for the sustained
action of mast cell-derived TNF during IgE-dependent biological responses.
J Exp Med 174: 103-107.
- D.T.W. Wong, A. Elovic, K. Matossian, N. Nagura, J. McBride, J.R. Gordon,
T.H. Rand, S.J. Galli, and P.F. Weller, 1991. Human eosinophils express TGF ß1. Blood 78: 2702-2707.
- B.K. Wershil, Z.-S. Wang, J.R. Gordon, and S.J. Galli, 1991. The recruitment
of neutrophils during IgE-dependent cutaneous late phase reactions in the
mouse is mast cell-dependent: partial inhibition of the reaction with antiserum
against TNF. J Clin Invest 87: 446-453.
- J.R. Gordon and S.J. Galli, 1990. Mast cells as a source of both pre-formed
and immunologically-inducible TNF. Nature 346: 274-276.
- J.R. Gordon and S.J. Galli, 1990. Phorbol 12-myristate-13-acetate-induced
development of functionally active mast cells in W/Wv but not SI/SId genetically
mast cell-deficient mice. Blood 75: 1637-1645.
- P.R. Burd, H.W. Rogers, J.R. Gordon, C.A. Martin, S. Jayaraman, S.D. Wilson,
A.M. Dvorak, S.J. Galli, and M.E. Dorf, 1989. IL-3-dependent and -independent
mast cells stimulated with IgE and antigen express multiple cytokines. J Exp
Med 170: 245-257.
- J.R. Gordon and D.J. McLaren, 1988. Immunological responses to Schistosoma
mansoni in guinea pigs vaccinated with radiation-attenuated cercariae. T cell
activation of macrophages for schistosomular killing. Immunol 63: 173-180.
- J.R. Gordon and D.J. McLaren, 1987. Immunity to Schistosomiasis mansoni in
guinea pigs vaccinated with radiation-attenuated cercariae. I. Humoral responses
against skin stage schistosomula. Clin Exp Immunol 67: 252-261.
- J.R. Gordon and J.R. Allen, 1987. Isolation and characterization of the salivary
antigens from the female tick, Dermacentor andersoni. Parasite Immunol 9:
337-352.
PATENTS
- A high affinity interleukin-8 analogue for use as an anti-inflammatory agent.
United States Patent and Transfer Office No 60/273,181; International Patent
Office No. PCT/CA02/00271. Inventors: John R. Gordon and Fang Li. Assignee,
University of Saskatchewan Technologies Inc. Filed Mar 1, 2002. Describes
a novel compound engineered, based on CXCL8, that displays remarkable antagonist
activities against the CXCR1 and CXCR2 in human, porcine, bovine, and cavean
systems.
- Protective vaccination against sensitization for allergies using very low
doses of allergen. U.S. Patent Transfer Office provisional number 60/182,185.
Inventor: John R. Gordon. Assignee, University of Saskatchewan Technologies
Inc. Filed Feb 14, 2000. Describes a means of inducing essentially complete
prophylactic vaccination against agents that would otherwise inevitably induce
allergic or Th2-dependent disease. Not licensed to date.
TRAINEES
Current trainees
Aarti Nayyar (BVSc), MSc/PhD in tolerization of asthma & allergy
Xixing Zhang (BVSc), MSc/Ph.D in chemokine immunopathology
PDF: Wenjun Wang (PhD), Roles of ELR-CXC chemokines in systemic endotoxemia
Past trainees (last 5 years)
- Fang Li (BSc, MSc, PhD), Visiting Research Scientist (presently, Head of
Immunology, Dalian Medical Univ, PRC), Engineering Novel Chemokine Antagonists.
2004.
- Fang Li (Bsc, MSc, PhD), post-doctoral fellowship, Development of a CXCRI/CXCRII
antagonist. 1999-2001.
- Christine Theoret (MSc, DVM), PhD in equine wound healing. (presently, Assist
Prof, University of Montreal). 1997-2000 (co-supervised with Dr. S. Barber)
- Jeff Caswell (MSc, DVM), PhD in role of CXCL8 in bovine neutrophilic inflammation.
(co-supervised with Dr. D. Middleton)
- Angela Schneider (BSc), MSc in regulation of allergic Th2 sensitization.
- Binbin Yue (BSc, MSc [Japan]), MSc in genetic regulation of cytokine production
- Kelly McClellan (BSc, DVM) MVSc in sarcoid tumor development (co-supervised
with Dr. S. Barber).
RESEARCH GRANT INFORMATION (last 5 years)
- CIHR operating grant. 2005-08. Mechanisms mediating tolerogenic dendritic cell therapy in allergic asthma . $ 338,175.
- NSERC Discovery Grant, 2005-09. ELR-CXC chemokines in bacterial inflammation . $145,000.
- Research contract with IL Therapeutics - Humanization of the CXCR1/CXCR2 antagonist bovine CXCL8 (3-73) K11R/G31P . Oct '04- Oct '05, $186,000.
- J. Marshall (Leader) and 7 others. AllerGEN CIHR National Centre of Excellence Strategic Grant. 2005-06. Functional consequences of allergic disease-associated polymorphisms in innate immune receptor systems . $80,000.- Natural Sciences and Engineering Research Council Equipment Grant. 2004.
Integrated intravital and in vitro imaging facility. $70,000 ($90,000 matching
from institutional resources). B. Singh, J.R. Gordon, V. Misra, J. Singh
-Saskatchewan Health Research Foundation (SHRF) Health Research Groups Facilitation
Grant. 2004-06. J.R. Gordon & P.A. Bretscher, for the University of Saskatchewan
Immunology Research Group. $100,000
-CIHR operating grant. Therapeutic immunomodulation in allergic lung disease/asthma.
2002-05, $218,000
-Sask Dept Agricult. Development of a therapeutic agent to ameliorate the
pathology of bacterial inflammation in cattle. 2002-03, $14,982
-Sask Lung Assoc (Canad Thoracic Soc review). Development of an effective
Th2-reversing allergen-adjuvant immunotherapy for treatment of allergic lung
disease. 2000-02, $60,000
-Agricult Devel Fund (Sask)/Alberta Agri Res Inst matching grant. Development
of an antagonist of the neutrophil chemoattractant IL-8 for pneumonic pasteurellosis
in cattle. 1999-01, $160,000
-Agricult Devel Fund (Sask). The role of interleukin-8 as a neutrophil chemoattractant
in bovine bronchopneumonia. 1996-98, $25,000
-Sask Lung Assoc. Effects of regular b2-agonist treatments on the pulmonary
early and late inflammatory responses to allergen challenge in allergic asthma
patients". J.R. Gordon & D. Cockcroft - 1997-98, $17,900
-Medical Research Council of Canada. The roles of mast cell-cytokine networks
in allergic pathophysiology. 1995-98, $231,000
COMMITTEE & OTHER WORK (national/international)
Member, CIHR Mucosal Immun. & Resp. Diseases Outcomes RFA review panel, '05
- Chair, organizing committee, Canadian Society for Immunol 2007 Annual Mtg ( Lake Louise )
- Member, Canadian Cystic Fibrosis Foundation Grant Review panel , 2004-
- Member, Canadian Allergy, Asthma & Immunol Fndt Fellowship Review Panel , 2004-
- Leader, Immunology Section, Canadian Swine Infectious Diseases Network (NSERC SIDNet)
- Scientific Advisory Panel, IL Therapeutics, Inc ( Innovation Place , Saskatoon ), '05-
-Saskatchewan Health Research Foundation Establishments Grants Review panel,
Chairman, 2004-06
-Canadian Society for Immunology: Secretary/Treasurer, 2003- ; meeting organizing
and student award committees,
-Canadian Institutes for Health Research: Immunology & Transplantation
review panel member, 2002-’03; invited member, 2002; MRC/CIHR grant
and scholarship applications reviewer (external), 1991-2002.
-American Association of Immunologists: primary reviewer, Journal of Immunology,
2000- ; ad hoc reviewer, 1988-'00.