Flow Cytometry User Group

  • Promotes the use of flow cytometry as a research tool to enhance research activity.
  • Supports the development of technical expertise and applications. 
  • Facilitates interactions among researchers in the life and material sciences.
  • Creates opportunities for collaborations, access to equipment, training, and multidisciplinary research.
Mission and Vision
  • Mission statement

The Flow Cytometry Group will promote the use of flow cytometry as a research tool by supporting the development of technical expertise and applications within the U of S research community.  We will address these objectives by facilitating interactions among researchers interested in human and animal health, biology, agriculture, toxicology, engineering and other disciplines and creating opportunities for collaborations, access to equipment, and multidisciplinary research based on flow cytometry. 

Multidisciplinary research involving flow cytometry is also conducive to advanced training of students from diverse backgrounds.  Scientific education with expertise in multiple fields allows graduate students and technical staff to explore new areas of research and become more competitive when seeking employment.

We will promote more efficient use of currently available instrumentation on campus and facilitate training of new users through workshops, user meetings, and information sessions. This will be achieved by establishing a virtual flow cytometry facility to facilitate communication among users and identifying gaps in currently available instrumentation, reagents, or expertise.  The Flow Cytometry Group will assist in developing strategies to address these deficiencies.

  • Vision statement

The Flow Cytometry Group envisions greater use of flow cytometry to enhance research activity and excellence at the University of Saskatchewan in all areas encompassing life and material sciences.

Training Sessions

Flow Cytometry Basics

Seminar: Flow Cytometry Basics

To provide information on the fluidics, optics and electronics of a flow cytometer using FACSCalibur as model. Issues regarding compensation and manual correction will be discussed. Data output and interpretation will be described. The same seminar content is offered on three separate dates.

  • Oct 21 2014, 1:30pm, location: MURRY 299
  • Oct 27 2014, 1:30pm, location: ARTS 104
  • Oct 30 2014, 1:30pm, location: ARTS 104

Sample Preparation and Experimental Design

To maximize information quality, experimental design and sample preparation are critical components of every flow cytometry experiment. This seminar addresses the basic considerations and procedures to ensure optimal sample preparation and discusses the necessary controls for effective data analysis. Strategies for reagent selection and experimental design for multi-colour flow cytometry are also addressed. The same seminar content is offered on three separate dates.

  • Nov 17 2014, 1:30pm, location: ARTS 104
  • Nov 20 2014, 1:30pm, location: ARTS 104
  • Nov 25 2014, 1:30pm, location: MURRY 299

Wet Lab with Flow Cytometer to Demonstrate Key Principles

This lab will introduce first-time users the works of a flow cytometer (FACSCalibur). Individuals will learn to set detectors, create histograms and dot plots using Cell Quest Pro software.  Forward and side scattering plots will be interpreted. Course dates are to be determined

High-speed Sorting of Cell Populations

Cell sorters have become a widespread and vital tool in biological and health science research. Their central purpose is to recover viable and homogeneous subpopulations of cells from a heterogeneous population. This seminar discusses the principles and limitations of cell sorting, and the practical considerations when designing an effective sorting experiment. Sample preparation, cell staining, fluorochrome selection, appropriate controls, and sample sorting and collection conditions will be discussed. Course dates are to be determined

DNA Analysis with ModFit LT

To provide information on quantification of DNA in cell cycling using mammalian cells as a model. The algorithms to calculate DNA quantity and statistical analysis on the fit to the model by ModFit will be discussed. Cancer-related examples will be provided. Course dates are to be determined

Cell Proliferation Studies with ModFit LT

Cell proliferation has historically been monitored by the incorporation of radionucleotides such as tritiated thymidine (3H-TdR) into newly synthesized DNA. In this seminar we focus on the use of the ModFit LT cell proliferation wizard feature to track cell division and identify proliferating cells. The use of various cell tracking dyes will be discussed with examples of cell proliferation studies to address specific research questions. Course dates are to be determined

Upcoming Meetings
Core Committee

  • Philip Griebel, DVM, PhD (Chair)

Tier I CRC Mucosal Immunology of the Neonate
Professor, School of Public Health
Research Fellow, VIDO-Intervac
University of Saskatchewan
Tel: 306-966-1542
Email: philip.griebel@usask.ca

  • Ildiko Badea, PhD (Vice Chair)

Associate Professor of Pharmacy
College of Pharmacy and Nutrition
University of Saskatchewan
Tel: 306-966-6349
Email:  ildiko.badea@usask.ca

Advisory committee

  • John R. Gordon, PhD

Professor, Department of Medicine
University of Saskatchewan
Tel: 306-966-1901
Email: john.gordon@usask.ca

  • Marko Kryworuchko, PhD

Adjunct Professor, School of Public Health
University of Saskatchewan
Tel: 306-966-7494
Email: Marko.Kryworuchko@usask.ca

  • Natasa Arsic, MSc.

Research Technician/ Flow Cytometry Lab Manager
VIDO-InterVac
University of Saskatchewan
Tel: 306-966-1556
Email: natasa.arsic@usask.ca

  • Deborah Michel, BSc (Secretary)

Research Technician
College of Pharmacy and Nutrition
University of Saskatchewan
Tel: 306-966-6348
Email: dlm137@mail.usask.ca

Members
Available Instrumentation
Analysers

FACScalibur, Dr. Badea, contact: ildiko.badea@usask.ca
two laser at 488 and 635nm,
emission filters 535/30, 585/42, 670LP and 661/16 nm
Location: D-Wing, Health Sciences Building

Alternate Contact: Deborah Michel - dlm137@mail.usask.ca

Sorters

Beckman Coulter MoFlo XDP Cell sorter is equipped with a 488nm Argon laser (Filters:529/28;625/26;575/25;670/30;785/62) and 633nm HeNe laser (670/30;785/62) and is located in the biosafety level-2 (BSL-2) laboratory space in VIDO A214. Additional filter set up is available.
Single cell suspensions can be characterized and sorted at 10-30,000 events per second based on two light scattering parameters (FSC, SSC) and up to seven fluorochrome colours.
Up to four distinct cell populations can be sorted simultaneously from a single cell suspension.

Dr. Griebel, contact: philip.griebel@usask.ca
Location: VIDO

Alternate Contact: Natasa Arsic - natasa.arsic@usask.ca

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Mo Flo Service Request Form
Scheduling and Billing

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