Dr. Anas El-Aneed
B.Sc. Pharm & Pharm. Chemistry (Teshreen University, Syria ), M.Sc. Pharmaceutics (Memorial University of Newfoundland), MBA (University of Saskatchewan), Ph.D. Biochemistry (Memorial University of Newfoundland)Assistant Professor of Pharmacy
Physicochemical Principles of Drugs (Undergraduate) and Analytical Mass Spectrometry (Graduate).
- Analytical mass spectrometry including tandem mass spectrometry using various ionization methods
- Developing novel qualitative and quantitative mass spectrometric-based analytical methods with current focus on lipid-based drug carriers and novel antineoplastic agents
- Substance abuse
Basic Science: three main streams are being evaluated, focusing on the use of mass spectrometry (MS) for the rapid analysis of analytes in both qualitative and quantitative applications.
Stream 1: Development of novel MS qualitative and quantitative methods: The goal is to establish fast, robust and sensitive analytical methods that will allow for the determination of the biological fate of novel lipid-based drug delivery agents. To date, our group has established the universal tandem mass spectrometric (MS/MS) pathways/fingerprints of over 40 gemini surfactants, used as drug delivery agents. Therefore, tested agents can be identified within any biological or environmental matrices. We have identified selected lead compounds within a formulation matrix or tissue culture lysates. Six different MS-based quantification methods (within tissue culture lysate) were developed namely, HPLC-MS/MS, Flow Injection Analysis (FIA-MS/MS) using 3 different low-resolution and high resolution MS instruments, Matrix Assisted Laser Desorption Ionization (MALDI)-MS and Desorption Electrospray Ionization (DESI)-MS/MS. To my knowledge, it is the first time a comparative analysis of 6 different MS-based quantification methods has been investigated. Our long term goal is to demonstrate that LC-MS/MS, perceived as the gold standard for quantification within the pharmaceutical industry, may be in many situations an inferior technology in comparison to other MS-based quantification methods.
Our future work is to utilize the developed qualitative and quantitative methods for assessing the cellular disposition of structurally different gemini surfactants. The main goal is to highlight the relationship between the toxicity of these compounds and the molecular structure, so that newer and safer compounds can be synthesized.
Stream 2. Ionization mechanism for MALDI-MS: we are evaluating the mass spectrometric behavior of antineoplastic agents using both ESI and MALDI. A unique ionization behavior, resulting in the formation of an unexpected positively charged [M-H]+ has been identified when using MALDI-MS. In contrast, the expected [M+H]+ were observed when using ESI. In addition, the MS/MS fragmentation patterns were dependent on the ionization methods. Understanding the mechanism which led to variations between the two ionization methods is necessary to develop qualitative/quantitative MS-based methods and to analyze structurally-related compounds.
Stream 3. Design of novel MALDI matrices: we are currently evaluating the use of novel structurally- modified nanoparticles as MALDI matrices. Our results indicate that our newly-designed nanoparticles are superior to conventional matrices when analyzing low molecular weight pharmaceuticals. In particular, analyte signal was enhanced while background noise [commonly observed with conventional matrices] was reduced.
Applied Health Research: We recently evaluated barriers to accessing health services by Injection Drug Users (IDUs) in the city of Saskatoon. Focus groups with injection drug users and service providers revealed that stigma/discrimination and lack of system and personal resources hindered IDUs access to services. Currently, we are evaluating the educational needs (knowledge and skills) of pharmacists in the area of substance abuse. We aim to eventually develop an educational program [focusing on substance abuse] for pharmacists so that appropriate care is provided by the most accessible health care provider within the Canadian health care system.
Mohammed-Saeid W, Buse J, Badea I, Verrall RE, and El-Aneed A. Mass spectrometric analysis of amino acid/di-peptide modified gemini surfactants used as gene delivery agents: Establishment of a universal mass spectrometric fingerprint. International Journal of Mass Spectrometry. 2012; 309: 182-191.
Mohammed-Saeid W, Michal D, El-Aneed A, Verrall R, Low N, , Badea A*. Development of lyophilized gemini surfactant-based lipoplexes: influence of lyophilization on the Structure, Activity andS tability of the Lipoplexes. Journal of Pharmacy & Pharmaceutical Sciences 2012; 15: 548-567.
Michel, D, Chitanda, J, Balogh R, Singh J, Das U, El-Aneed A, Dimmock J, Verrall R, Badea, I. Design and evaluation of cyclodextrin-based delivery systems to incorporate poorly soluble curcumin analogs for the treatment of melanoma. Eur J Pharm Biopharm. 2012; 81: 548-556.
Buse J, Badea I, Verrall RE, and El-Aneed A. Tandem mass spectrometric analysis of novel diquaternary ammonium gemini surfactants and their bromide adducts in electrospray-positive ion mode ionization. Journal of Mass Spectrometry. 2011; 49: 1060-70.
Lang K, El-Aneed A, Berenbaum S, Dell CA, Wright J, Teed McKay Z. Qualitative Assessment of Crisis Services among Persons using Injection Drugs in the City of Saskatoon. Journal of Substance Use. 2011:1-9 (on line).
Buse J and El-Aneed A. Properties, Engineering and Applications of Lipid-based Nanoparticle Drug Delivery Systems: Current Research and Advances. Nanomedicine. 2010; 5: 1237-1260.
Banoub J, El-Aneed A, Cohen A, and Joly N. Structural Investigation of Bacterial Lipopolysaccharides by Mass Spectrometry and Tandem Mass Spectrometry. Mass Spectrometry Reviews. 2010; 29: 606-650.
Buse J, Badea I, Verrall RE, and El-Aneed A. Tandem Mass Spectrometric Analysis of the Novel Gemini Surfactant Nanoparticle Families G12-s and G18:1-s. Spectroscopy Letters. 2010; 29: 447-457.
El-Aneed A, Cohen A, and Banoub J. Basics of Mass Spectrometry: Ionization Methods, Mass analyzers and the Development of Q-ToF Tandem Hybrid Instruments. Applied Spectroscopy Reviews. 2009; 44: 210–230.
El-Aneed A, Alaghehbandan R, Gladeny N, MacDonald D and Fischer B. Prescription Drug Abuse and Methods of Diversion: The Potential Role of a Pharmacy Network. Journal of Substance Use. 2009; 14: 75–83.
El-Aneed A, Banoub J, Boullanger P, Lafont D and Koen-Alonso M. Establishment of mass spectrometric fingerprints of novel synthetic cholesteryl neoglycolipids: the presence of a unique C-Glycoside species during electrospray ionization and during collision induced dissociation tandem mass spectrometry. J. Am. Soc. Mass Spectrom. 2007; 18: 294-310.