Surface Plasmon Resonance

Surface plasmon resonance (SPR) is an optical phenomenon arising in thin metal films under conditions of total internal reflection. This phenomenon produces a sharp dip in the intensity of reflected light at a specific angle (called the resonant angle). This resonant angle depends on several factors, including the refractive index of the medium (refractive index is directly correlated to the concentration of dissolved material in the medium) close to the non-illuminated side of the metal film. By keeping other factors constant, SPR is used to measure the change in the concentration of molecules in the surface layer of solution in contact with the sensor surface.

BIACore-X Technology: The BIACore-X SPR uses a continuous flow technology to monitor the progress of biomolecular interactions in real time. By monitoring in real time, the BIACore-X system can be used to determine a variety of information for biomolecules and their interactions, including:

How the Instrument Works: (See BIACore webpage for details) One interactant, referred to as the ligand, is immobilized (different immobilization schemes are available for different types of biomolecules) on the sensor surface, , and forms one wall of a micro-flow cell. Solution containing the other interactant(s), referred to as the analyte(s), flows continuously over the sensor surface. As the analyte(s) bind to the ligand, the resonant angle changes (due to a change in refractive index of the medium) and a response is registered. Results are presented as a sensogram, which is a plot of changes in resonance signal as a function of time.

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