Pickering, Ingrid

Professor & Canada Research Chair

Ingrid Pickering

Department of Geological Sciences
114 Science Place
Saskatoon, Saskatchewan, Canada
S7N 5E2

Phone: (306) 966-5706

Email: ingrid.pickering@usask.ca

Website: http://homepage.usask.ca/~inp449/

Academic Degrees

  • B.A. (Cambridge), Ph.D. (London)

Current Teaching

  • GEOL 851.3 Synchrotron Hard X-ray Absorption Spectroscopy
  • GEOL 451.3 Synchrotron X-ray Absorption Spectroscopy
  • SYNC 801.3 Introduction to Synchrotron Health Research

Current and Recent Research Projects

  • Selenium in a cold freshwater environment
  • Molecular mechanisms of toxicity of mercury and selenium
  • Synchrotron speciation and imaging of toxic metals and heavier elements



MacDonald, T. C., S. Nehzati, N. J. Sylvain, A. K. James, M. Korbas, S. Caine, I. J. Pickering, G. N. George and P. H. Krone. Phenylthiourea alters toxicity of mercury compounds in zebrafish larvae. Journal of Inorganic Biochemistry151, 10–17 (2015) DOI: 10.1016/j.jinorgbio.2015.07.003

MacDonald, T. C., M. Korbas, A. K. James, N. J. Sylvain, M. J. Hackett, S. Nehzati, P. H. Krone, G. N. George and I. J. Pickering. Interaction of mercury and selenium in the larval stage zebrafish vertebrate model. Metallomics7, 1247-1255 (2015). DOI: 10.1039/c5mt00145e. Inside front cover article

Hackett, M. J., S. E. Smith, S. Caine, H. Nichol, G. N. George, I. J. Pickering and P. G. Paterson, Novel bio-spectroscopic imaging reveals disturbed protein homeostasis and thiol redox with protein aggregation prior to hippocampal CA1 pyramidal neuron death induced by global brain ischemia in the rat. Free Radical Biology and Medicine89, 806–818 (2015). DOI: 10.1016/j.freeradbiomed.2015.08.029.

Fleming, D. E. B., J. W. Groves, M. R. Gherase, G. N. George, I. J. Pickering, O. Ponomarenko, G. Langan, J. E. Spallholz, M. Alauddin, H. Ahsan, S. Ahmed and P. F. La Porte. Soft tissue measurement of arsenic and selenium in an animal model using portable X-ray fluorescence. Radiation Physics and Chemistry116, 241-247 (2015). DOI: 10.1016/j.radphyschem.2015.06.007

Foust, R. D., A.-M. Bauer, M. Costanza-Robinson, D. W. Blinn, R. C. Prince, I. J. Pickering and G. N. George. Arsenic transfer and biotransformation in a fully characterized freshwater food web. Coordination Chemistry Reviews, 306(2), 558–565 (2016). DOI: 10.1016/j.ccr.2015.03.005

Swanston, T., T. Varney, I. Coulthard, G. N. George, I. J. Pickering, R. Murphy and D. M. L. Cooper. Synchrotron X-ray fluorescence imaging evidence of biogenic mercury identified in a burial in colonial Antigua, Journal of Archaeological Science58, 26-30 (2015). DOI: 10.1016/j.jas.2015.03.006

Choudhury, S., J. Hormes, D. N. Agyeman-Budu, A. R. Woll, G. N. George, I. Coulthard, and I. J. Pickering. Application of a spoked channel array to confocal X-ray fluorescence imaging and X-ray absorption spectroscopy of medieval stained glass (from themed collection Synchrotron radiation in art and archaeology). Journal of Analytical Atomic Spectrometry30, 759-766 (2015). DOI: 10.1039/C4JA00389F

Choudhury, S., J. K. Thomas, N. J. Sylvain, O. Ponomarenko, R. A. Gordon, S. M. Heald, D. M. Janz, P. H. Krone, I. Coulthard, G. N. George and I. J. Pickering. Selenium preferentially accumulates in the eye lens following embryonic exposure: a confocal X-ray fluorescence imaging study. Environmental Science & Technology, 49(4), 2255–2261 (2015). DOI: 10.1021/es503848s

Hackett, M. J., C. J. Britz, P. G. Paterson, H. Nichol, I. J. Pickering and G. N. George. In situ biospectroscopic investigation of rapid ischemic and postmortem induced biochemical alterations in the rat brain. ACS Chemical Neuroscience6(2), 226–238 (2015). DOI: 10.1021/cn500157j 

Pushie, M. J., B. R. Pratt, T. C. MacDonald, G. N. George and  I. J. Pickering. Evidence for biogenic copper (hemocyanin) in the Middle Cambrian arthropod Marrella from the Burgess Shale. Palaios29(10), 512-524 (2014). DOI: 10.2110/palo.2014.073

Ponomarenko, O., M. R. Gherase, M. S. LeBlanc, C.-Y. Kim, E. D. Desouza, M. J. Farquharson, F. E. McNeill, S. Nehzati, I. J. Pickering, G. N. George and D. E. B. Fleming. Synchrotron XAS analysis of arsenic chemical speciation in human nail clippings. Environmental Chemistry 11(6) 632-643 (2014). DOI: 10.1071/EN13240

Alaverdashvili, M., M. J. Hackett, I. J. Pickering and P. G. Paterson. Laminar-specific distribution of zinc: Evidence for presence of Layer IV in forelimb motor cortex in the rat. NeuroImage 103, 502-510 (2014). DOI: 10.1016/j.neuroimage.2014.08.046. Cover article

George, G. N., M. J. Hackett, M. Sansone, M. Gorbaty, S. R. Kelemen, R. C. Prince, H. H. Harris and I. J. Pickering. Long-range chemical sensitivity in the sulfur K-edge X-ray absorption spectra of substituted thiophenes. The Journal of Physical Chemistry Part A 118(36), 7796-7802 (2014). DOI: 10.1021/jp505766f

Janz, D. M., K. Liber, I. J. Pickering, C. I. E. Wiramanaden, S. A. Weech, M. Gallego-Gallegos, M. K. Driessnack, E. D. Franz, M. M. Goertzen, J. Phibbs, J. J. Tse, K. T. Himbeault, E. L. Robertson, C. Burnett-Seidel, K. England and A. Gent. Integrative assessment of selenium speciation, biogeochemistry and distribution in a northern coldwater ecosystem. Integrated Environmental Assessment and Management, 10(4), 543-554 (2014). DOI: 10.1002/ieam.1560.  Received Best Paper for 2014 from integrated Environmental Assessment and Management (announcements:  DOI:  10.1002/ieam.1667)  

Pushie, M. J., I. J. Pickering, M. Korbas, M. J. Hackett and G. N. George. Elemental and chemically specific X-ray fluorescence imaging of biological systems. Chemical Reviews, 114(17), 8499-8541 (2014). DOI: 10.1021/cr4007297

Bañuelos, G. S., I. Arroyo, I. J. Pickering, S. I. Yang and J. L. Freeman. Selenium biofortification of broccoli and carrots grown in soil amended with Se-enriched hyperaccumulator Stanleya pinnata. Food Chemistry, 166(1), 603-608 (2015).
DOI: 10.1016/j.foodchem.2014.06.071.

Zeini Jahromi, E., J. Gailer, I. J. Pickering and G. N. George. Structural characterization of Cd2+ complexes in solution with DMSA and DMPS. Journal of Inorganic Biochemistry, 136, 99-106 (2014). DOI: 10.1016/j.jinorgbio.2013.10.025.

Truong, H-Y. T., Y-W. Chen, M. Saleh, S. Nehzati, G. N. George, I. J. Pickering and N. Belzile. Proteomics of Desulfovibrio desulfuricans and X-ray absorption spectroscopy to investigate mercury methylation in the presence of selenium. Metallomics 6(3), 465-475 (2014).
DOI: 10.1039/C3MT00323J. Metallomics HOT article

Pushie, M. J., K. H. Nienaber, K. L. Summers, J. J. H. Cotelesage, O. Ponomarenko, H. K. Nichol, I. J. Pickering and G. N. George. The solution structure of the copper clioquinol complex. Journal of Inorganic Biochemistry 133, 50-56 (2014). DOI: 10.1016/j.jinorgbio.2014.01.003.

Franz, E. D., C. I. E. Wiramanaden, M. Gallego-Gallegos, J. J. Tse, J. Phibbs, D. M. Janz, I. J. Pickering and K. Liber. An in situ assessment of selenium bioaccumulation from water-, sediment-, and dietary-exposure pathways using caged Chironomus dilutus larvae.  Environmental Toxicology and Chemistry, 32(12), 2836-2848 (2013). DOI: 10.1002/etc.2382

Korbas, M., B. Lai, S. Vogt, S. C. Gleber, C. Karunakaran, I. J. Pickering, P. H. Krone and G. N. George. Methylmercury targets photoreceptor outer segments. ACS Chemical Biology, 8(10), 2256-2263 (2013). DOI: 10.1021/cb4004805. 

Hackett, M.J., F. Borondics, D. Brown, C. J. Hirschmugl, S. E. Smith, P. G. Paterson, H. Nichol, I. J. Pickering and G. N. George. A sub-cellular biochemical investigation of Purkinje neurons using synchrotron radiation Fourier transform infrared spectroscopic imaging with a focal plane array detector. ACS Chemical Neuroscience, 4(7), 1071-1080 (2013). DOI: 10.1021/cn4000346. Cover article

Schmidt, R., P. Tantoyotai, S. C. Fakra, M. A. Marcus, S. I. Yang, I. J. Pickering, G. S. Bañuelos, K. R. Hristova and John L. Freeman. Selenium biotransformations in an engineered aquatic ecosystem for bioremediation of agricultural wastewater via brine shrimp production. Environmental Science and Technology, 47(10), 5057-5065 (2013). DOI: 10.1021/es305001n

Gallego-Gallegos, M., L. E. Doig, J. J. Tse, I. J. Pickering and K. Liber. Bioavailability, toxicity and biotransformation of selenium in midge (Chironomus dilutus) larvae exposed via water or diet to elemental selenium particles, selenite, or selenized algae. Environmental Science and Technology, 47(1), 584-592 (2013). DOI: 10.1021/es300828r

George, G. N., I. J. Pickering, M. J. Pushie, K. Nienaber, M. J. Hackett, I. Ascone, B. Hedman, K. O. Hodgson, J. B. Aitken, A. Levina, C. Glover and P. A. Lay. X-ray induced photo-chemistry and X-ray absorption spectroscopy of biological samples. Journal of Synchrotron Radiation,19(6), 875-886 (2012). DOI: 10.1107/S090904951203943X

Cotelesage, J. J. H., P. Grochulski, I. J. Pickering, G. N. George and M. N. Fodje. X-ray absorption spectroscopy at a protein crystallography facility ‒ the Canadian Light Source Beamline 08B1-1. Journal of Synchrotron Radiation19(6), 887-891 (2012). DOI: 10.1107/S090904951204023X

Bañuelos, G. S., S. S. Walse, S. I. Yang, I. J. Pickering, S. C. Fakra, M. A. Marcus and J. L. Freeman. Quantification, localization and speciation of selenium in seeds of canola and two mustard species compared to seed-meals produced by hydraulic press. Analytical Chemistry84(14), 6024–6030.  DOI: 10.1021/ac300813e.

Cotelesage, J. J. H., M. J. Pushie, P.  Grochulski, I. J. Pickering and G. N. George. Metalloprotein active site structure determination: synergy between X-ray absorption spectroscopy and X-ray crystallography. Journal of Inorganic Biochemistry115, 127-137 (2012).  DOI:10.1016/j.jinorgbio.2012.06.019

Tse, J. J., M. Gallego-Gallegos, E. D. Franz, K. Liber and I. J. Pickering. Selenium speciation and localization in chironomids from lakes receiving treated metal mine effluent. Chemosphere89(3), 274-279 (2012). 
DOI: 10.1016/j.chemosphere.2012.04.036.

Bañuelos, G. S., C. Stushnoff, S. S. Walse, T. Zuber, S. I. Yang, I. J. Pickering, and J. L. Freeman. Biofortified, selenium enriched, fruit and cladode from three Opuntia Cactus pear cultivars grown on agricultural drainage sediment for use in nutraceutical foods. Food Chemistry,135, 9-16 (2012).  DOI:10.1016/j.foodchem.2012.04.021.

Hackett, M. J., S. E. Smith, P. G. Paterson, H. Nichol, I. J. Pickering and G. N. George. X-ray absorption spectroscopy at the sulfur K-edge: a new tool to investigate the biochemical mechanisms of neurodegeneration. ACS Chemical Neuroscience 3(3), 178–185 (2012). DOI: 10.1021/cn200097s.

Pushie, M.J., L. Zhang, I. J. Pickering and G. N. George. The fictile coordination chemistry of cuprous-thiolate sites in copper chaperones.Biochimica et Biophysica Acta - Bioenergetics817(6) 938-947 (2012). 
DOI: 10.1016/j.bbabio.2011.10.004.

Korbas, M., T. C. MacDonald, I. J. Pickering, G. N. George and P. H. Krone. Chemical form matters: differential accumulation of mercury following inorganic and organic mercury exposures in zebrafish larvae. ACS Chemical Biology 7(2) 410-419 (2012).  DOI: 10.1021/cb200287c.

Yang, S.I., J. R. Lawrence, G. D. W. Swerhone and I. J. Pickering. Biotransformation of selenium and arsenic in multi-species biofilm.Environmental Chemistry8(6), 543-551 (2011). DOI: 10.1071/EN11062.