BEHAVIORAL AND PHYSIOLOGICAL RESPONSES OF HORSES TO INITIAL TRAINING: THE COMPARISON BETWEEN PASTRUE VERSUS STALLED HORSES

E. Rivera; S. Benjamin; A. Morrison; L. Callender; B. D. Nielsen and A. J. Zanella. Michigan State University, East Lansing, MI.

Pasture kept horses have the ability to interact socially and are exposed to a richer environment but have less contact with humans. Horses kept in stalls have greater exposure to human contact however they lack social interaction. There are evidences, for several species, that learning ability may be impaired in animals housed in social isolation or barren environment. In the present work the behavior and physiological responses to initial training was monitored in stall (S) and pasture (P) kept horses. Sixteen, 2 year old Arabian horses were involved in the present study. Twelve horses were subjected to a standardized "training procedure" (6P & 6S) and 4 (2P & 2S) animals were selected as control (C). Training was carried out by two trainers who handled both pasture and stall kept horses. The horses, which were never ridden before, were kept in pasture or in individual stalls for three months prior to this experiment. Behavioral observation assessing the interactions between the trainer and the horse in training were collected using video recorder and data was analyzed using a behavior observation software. Measures of plasma cortisol were monitored by radioimmunoassay in blood samples collected by jugular puncture in training days 1, 7, 21 and 28. Samples were collected prior to training (basal), immediately post-training (pt), 15 minutes post training (pt15) and 60 minutes post-training (pt60). During the first training day, horses were haltered and lead to the round training pen where they were released. The horse was caught and gradually the trainer began to get the horse accustomed to handling, being saddled, mounted and ridden within an average of 30 minutes. The horses were encouraged to walk, trot and canter in each direction. Control horses were released into the round pen and left to explore the environment for 30 minutes. Additional tasks were incorporated during days 7, 21 and 28 of training protocol. Horses kept in stalls showed significantly higher frequency of behavioral patterns associated with resistance to training than pasture kept horses. Bucking and jumping (S=3.83 q 10.81SEM and P=1.09 q 10.30SEM, p<0.05) head tossing (S=5.17 q 11.29SEM and P=2.04 q 1.74SEM ) tucking of tail (S=6.08 q 11.35SEM and P=2.09 q 1.526SEM ) were performed more by stall housed horses. Stall housed horses ran for longer periods in the training pen than pasture kept horses (S=7.10 q 11.04SEM and P=2.21 q 1.753SEM ). Increase in salivary cortisol in response to training, tended to be higher in stall housed horses for all the samples taken in the four experimental days, but the difference only approached significance (p=0.08, df, F-2.62) in samples collected immediately post-training (pt). The highest increase in plasma cortisol levels was observed in the first training day and the lowest was observed at the day 28. The difference among training days was significant (df 3; f=7.16; p=0.0004). Differences between basal cortisol levels and post-training levels were not evident for control horses in days 7, 21 and 28 post-training. Effective training may be accomplished in rather short time using humane techniques. Housing conditions seems to have a significant effect on the behavioral responses of horses to training but no effect on plasma cortisol levels. These results may generate recommendations that could be given to horse owners on which training technique and housing conditions that would maximize the success of training procedures.