University of Saskatchewan

Western College of Veterinary Medicine

Honaramooz, Ali

Professor
Veterinary Biomedical Sciences
Office Location: WCVM 2255
Phone: 306-966-7355
Fax: 306-966-7376
Email: ali.honaramooz@usask.ca


Academic Credentials


Teaching and Clinical Areas

Dr. Honaramooz's teaching responsibilities include veterinary anatomy and veterinary histology in the DVM program and reproductive physiology in the graduate student program.


Research Interests

Dr. Honaramooz's research interests are in reproductive biology and technology, with emphasis on the study and manipulation of spermatogenesis and spermatogonial stem cells. His research team has developed the technique for germ cell (spermatogonial) transplantation in farm animals, a procedure in which testis cells are harvested from a fertile donor male and microinjected into the seminiferous tubules of an infertile recipient. This system provides a unique functional assay to determine stem cell potential of a given population of donor germ cells.

Studies are underway to improve the outcome of germ cell transplantation to be used as a viable alternative approach to generate transgenic farm animals. Honaramooz and his team members have also established the technique for testis tissue xenografting, a model system that allows the progression of spermatogenesis from a variety of mammalian species in a laboratory mouse.

Using this system, it has now become feasible to produce sperm even from newborn domestic and wild animals in a host mouse. Testis tissue xenografting has opened a new avenue of research in reproductive biology, and the Honaramooz research team's current studies are aimed at introducing this technology as a novel tool for


Publications

Honaramooz A. 2012. "Cryopreservation of testicular tissue." In Cryopreservation: Book 1. Ed: Katkov I. InTech Open Access Publisher (ISBN 979-953-307-743-6). In press.

Honaramooz A, Yang Y. 2011. "Recent advances in application of male germ cell transplantation in farm animals." Veterinary Medicine International, Doi; 10.4061/2011/657860.

Abbasi S, Honaramooz A. 2011. "Xenografting of testis tissue from bison calf donors into recipient mice as a strategy for salvaging genetic material." Theriogenology. 76: 607-614.

Yang Y, Honaramooz A. 2011. "Efficient purification of neonatal porcine gonocytes with Nycodenz and differential plating." Reproduction, Fertility and Development. 23: 496-505.

Zeng W, Baumann C, Schmidtmann A, Honaramooz A, Tang L, Bondareva A, Dores C, Fan T, Xi S, Geiman T, Rathi R, de Rooij D, De La Fuente R, Muegge K, Dobrinski I. 2011. "Lymphoid-specific helicase (HELLS) is essential for meiotic progression in mouse spermatocytes." Biology of Reproduction. 84: 1235-1241.

Abbasi S, Honaramooz A. 2011. "The number of grafted fragments affects the outcome of testis tissue xenografting from piglets into recipient mice." Veterinary Medicine International. Doi: 10.4061/2011/6686570.

Yang Y, Yarahmadi M, Honaramooz A. 2010. "Development of novel strategies for the isolation of piglet testis cells with a high proportion of gonocytes." Reproduction, Fertility and Development. 22: 1057-1065.

Abbasi S, Honaramooz A. 2010. "The effects of recipient mouse strain, gender and gonadal status on the outcome of testis tissue xenografting." Reproduction, Fertility and Development. 22: 1279-1286.

Turner R, Rathi R, Honaramooz A, Zeng W, Dobrinski I. 2010. "Xenografting restores spermatogenesis to cryptorchid testicular tissue but does not rescue the phenotype of idiopathic testicular degeneration in the horse (Equus caballus)." Reproduction, Fertility and Development. 22(4): 673-683.

Yang Y, Steeg J, Honaramooz A. 2010. "The effects of tissue sample size and media on short-term hypothermic preservation of porcine testis tissue." Cell and Tissue Research. 340(2): 397-406.

Yang Y, Honaramooz A. 2010. "Effects of medium and hypothermic temperatures on preservation of isolated porcine testis cells." Reproduction, Fertility and Development. 22(3): 523-532.

Abrishami M, Abbasi S, Honaramooz A. 2010. "The effect of donor age on progression of spermatogenesis in canine testicular tissue after xenografting into immunodeficient mice." Theriogenology. 73(4): 512-522.

Abrishami M, Anzar M, Yang Y, Honaramooz A. 2010. "Cryopreservation of immature porcine testis tissue to maintain its developmental potential after xenografting into recipient mice." Theriogenology. 73(1): 86-96.

Honaramooz A, Li MW, Penedo CT, Meyers SA, Dobrinski I. 2004. "Accelerated maturation of primate testis by xenografting into mice." Biology of Reproduction. 70: 1500-1503.

Honaramooz A, Behboodi E, Megee SO, Overton SA, Galantino-Homer H, Echerlard Y. Dobrinski I. 2003. "Fertility and germline transmission of donor haplotype following germ cell transplantation in immuno-competent goats." Biology of Reproduction. 69: 1260-1264.

Honaramooz A, Snedaker A, Boiani M, Schöler H, Dobrinski I, Schlatt S. 2002. "Sperm form neonatal mammalian testes grafted in mice." Nature. 418: 778-781.

Honaramooz A, Megee SO, Dobrinski I. 2002. "Germ cell transplantation in pigs." Biology of Reproduction. 66: 21-28.